Anti-FAK (phospho Y397) antibody
| Name | Anti-FAK (phospho Y397) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39967 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human, Drosophila, Rat, Chicken, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human FAK, phosphorylated at Y397 |
| Blocking Peptide | Mouse FAK (phospho Y397) peptide |
| Description | Rabbit Polyclonal |
| Gene | PTK2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Performed under reducing conditions.
ICC/IF image of ab39967 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab39967, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-FAK (phospho Y397) antibody (ab39967) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Mouse FAK (phospho Y397) peptide (ab40145) at 1 µg/mlLane 3 : A431 (Human epithelial carcinoma cell lines) EGF Stimulated Whole Cell Lysate with Mouse FAK peptide (ab53601) at 1 µg/mlLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionPerformed under reducing conditions.
Product References
Regulation of the fate of human mesenchymal stem cells by mechanical and - Regulation of the fate of human mesenchymal stem cells by mechanical and
Kuo SW, Lin HI, Ho JH, Shih YR, Chen HF, Yen TJ, Lee OK. Biomaterials. 2012 Jul;33(20):5013-22.
Integrin betanu-mediated phagocytosis of apoptotic cells in Drosophila embryos. - Integrin betanu-mediated phagocytosis of apoptotic cells in Drosophila embryos.
Nagaosa K, Okada R, Nonaka S, Takeuchi K, Fujita Y, Miyasaka T, Manaka J, Ando I, Nakanishi Y. J Biol Chem. 2011 Jul 22;286(29):25770-7.