Anti-FAK (phospho Y397) antibody [EP2160Y]

• Supplier: Abcam
• Catalog: ab81298
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP2160Y
• Applications: ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FAK
• Blocking Peptide: FAK peptide
• Description: Rabbit Monoclonal
• Gene: PTK2
• Conjugate: Unconjugated

Product images

All lanes : Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/2000 dilution (purified)Lane 1 : Untreated mouse brainLane 2 : Mouse brain treated with alkaline phosphataseLysates/proteins at 10 µg per lane.SecondaryHRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/1000 dilution (purified)Lane 1 : Untreated rat brainLane 2 : Rat brain treated with alkaline phosphataseLysates/proteins at 10 µg per lane.SecondaryHRP conjugated goat anti-rabbit IgG (H+L) at 1000 µg

All lanes : Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/2000 dilution (unpurified)Lane 1 : human brain tissue lysates, untreated.Lane 2 : human brain tissue lysates treated with AP.Lane 3 : rat brain tissue lysates, untreated.Lane 4 : rat brain tissue lysates treated with AP.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit. at 1/2000 dilution

ICC/IF image of unpurified ab81298 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81298, 10µg/ml) overnight at +4°C in PBS containing 1% BSA and 0.1% tween. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Unpurified ab81298 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (in water soluble emulsion) (ab120429), by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (in water soluble emulsion), as described in literature.The cells were incubated at 37°C for 5 minutes in media containing different concentrations of ab120429 (anandamide (in water soluble emulsion)) in water, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81298 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.

Unpurified ab81298 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (ethanol solution) (ab120087), by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (ethanol solution), as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120087 (anandamide (ethanol solution)) in ethanol, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81298 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.

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