All lanes : Anti-FAK (phospho Y861) antibody (ab200811) at 1/1000 dilutionLane 1 : Extracts of primary CEF cells expressing FAK with phosphopeptide immunogenLane 2 : Extracts of primary CEF cells expressing FAK with a generic phosphotyrosine-containing peptideLane 3 : Extracts of primary CEF cells expressing FAK with a non-phosphorylated peptide corresponding to the immunogenLane 4 : Extracts of primary CEF cells expressing FAKSecondaryGoat F(ab’)2 anti-rabbit IgG HRPdeveloped using the ECL technique