Anti-FANCM antibody
| Name | Anti-FANCM antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab95014 |
| Prices | $384.00 |
| Sizes | 20 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Orangutan, Bat, Elephant |
| Antigen | A region within N terminal amino acids 1-50 (MSGRQRTLFQ TWGSSISRSS GTPGCSSGTE RPQSPGSSKA PLPAAAEAQL) of Human FANCM (NP_065988 |
| Description | Rabbit Polyclonal |
| Gene | FANCM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-FANCM antibody (ab95014) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
ab95014, at 0.4 µg/ml, detecting FANCM in HeLa whole cell lysate by WB following IP. Lane 1: ab95014 at 10 µg/mg of lysate Lane 2: IP with an antibody which recognizes a downstream epitope of FANCM Lane 3: control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection: Chemiluminescence with an exposure time of 10 seconds
ab95014 staining FANCM in HeLa cells by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in 2% Triton X-100 prior to blocking in 7% serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in 0.1% Tween 20 in PBS and incubated with the sample for 17 hours at 4°C. The secondary antibody was Alexa Fluor® 555 conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.See Abreview