ab103719 stained HCT116 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103719 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-FBXL15 antibody (ab103719) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg
Anti-FBXL15 antibody (ab103719) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg
ab103719 at 1/50 dilution staining FBXL15 by immunohistochemistry in formalin fixed and paraffin embedded Human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.