Anti-FDFT1 antibody [EPR16481]

• Supplier: Abcam
• Catalog: ab195046
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16481
• Applications: IP IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human FDFT1 aa 150-300
• Description: Rabbit Monoclonal
• Gene: FDFT1
• Conjugate: Unconjugated

Product images

All lanes : Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilution + HepG2 cell lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat kidney lysateLane 3 : Rat spleen lysateLane 4 : RAW264.7 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

Immunohistochemical analysis of paraffin-embedded human sebaceous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

FDFT1 was immunoprecipitated from HepG2 whole cell extract with ab195046 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab195046 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HepG2 whole cell extract (Input) 10 µg. Lane 2: ab195046 IP in HepG2 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195046 in HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.