Anti-FEN1 antibody [4E7]
| Name | Anti-FEN1 antibody [4E7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab462 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4E7 |
| Applications | FC WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus-infected Sf-9 cells |
| Description | Mouse Monoclonal |
| Gene | FEN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab462 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab462, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18408_ab462-3-ab462FC.jpg)
Overlay histogram showing HeLa cells stained with ab462 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab462, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab462 detecting FEN1 (green) in Hela Cells by ICC/IF. Cells were counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.See Abreview
ab462 (2µg/ml) staining FEN1 in human duodenum sing an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining .Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
Human Fanconi anemia complementation group a protein stimulates the 5' flap - Human Fanconi anemia complementation group a protein stimulates the 5' flap
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Extracts of proliferating and non-proliferating human cells display different - Extracts of proliferating and non-proliferating human cells display different
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Mitochondrial base excision repair of uracil and AP sites takes place by - Mitochondrial base excision repair of uracil and AP sites takes place by
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Effect of polyamine deficiency on proteins involved in Okazaki fragment - Effect of polyamine deficiency on proteins involved in Okazaki fragment
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