FEN1

Gene Symbol FEN1
Entrez Gene 2237
Alt Symbol FEN-1, MF1, RAD2
Species Human
Gene Type protein-coding
Description flap structure-specific endonuclease 1
Other Description DNase IV|FEN-1|flap endonuclease 1|maturation factor 1|maturation factor-1
Swissprots P39748
Accessions AAB32522 AAC23394 AAM74238 EAW73972 P39748 AK301743 BAG63206 AK312761 BAG35627 BC000323 AAH00323 BM450370 BP244711 BT019524 AAV38331 CN290841 CR536562 CAG38799 L37374 AAA91331 X76771 CAA54166 NM_004111 NP_004102
Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {ECO:0000255|HAMAP-Rule:MF_03140, ECO:0000269|PubMed:10744741, ECO:0000269|PubMed:11986308, ECO:0000269|Pub
Subcellular Location Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Top Pathways DNA replication, Base excision repair, Non-homologous end-joining

FEN-1 Antibody - 2746 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and HeLa cells using FEN-1 Antibody.

FEN-1 (B-4) - sc-28355 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FEN-1 (B-4): sc-28355. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

FEN-1 (C-16) - sc-8795 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FEN-1 (C-16): sc-8795. Western blot analysis of FEN-1 expression in HeLa (A), Jurkat (B) and NIH/3T3 (C) nuclear extracts and Y79 (D) and PC-12 (E) whole cell lysates.

FEN-1 (N-17) - sc-8794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

FEN-1 (H-300) - sc-13051 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FEN-1 (H-300): sc-13051. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

FEN-1 (4E7) - sc-56675 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
FEN-1 (4E7): sc-56675. Western blot analysis of FEN-1 expression in HeLa (A), Jurkat (B), Y79 (C), A-431 (D), MCF7 (E) and U-937 (F) nuclear extracts.

Anti-FEN1 antibody - ab153825 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-FEN1 antibody (ab153825) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-FEN1 antibody - ab64908 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FEN1 antibody (ab64908) at 1/500 dilutionLane 1 : extracts from Jurkat cellsLane 2 : extracts from 293 cellsLane 3 : extracts from HUVEC cellsLane 4 : extracts from HUVEC cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-FEN1 antibody - ab464 from Abcam

Prices $359.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Anti-FEN1 antibody [4E7] - ab462 from Abcam

Prices $400.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4E7
Overlay histogram showing HeLa cells stained with ab462 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab462, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-FEN1 antibody [7H8-F4-C11] - ab190633 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7H8-F4-C11
All lanes : Anti-FEN1 antibody [7H8-F4-C11] (ab190633) at 1/1000 dilutionLane 1 : CHO-K1 cell lysateLane 2 : Raji cell lysateLane 3 : Raw246.7 cell lysateLane 4 : C6 cell lysateLane 5 : PC-12 cell lysateLane 6 : COS7 cell lysateLane 7 : 3T3 cell lysateLane 8 : HeLa cell lysateLane 9 : Jurkat cell lysate

Anti-FEN1 antibody [EPR4459(2)] - ab133311 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4459(2)
All lanes : Anti-FEN1 antibody [EPR4459(2)] (ab133311) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-FEN1 antibody [EPR4460(2)] - ab109132 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4460(2)
All lanes : Anti-FEN1 antibody [EPR4460(2)] (ab109132) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : Y79 cell lysateLane 4 : Daudi cell lysateLysates/proteins at 10 µg per lane.

Anti-FEN1 Antibody - LS-C20063 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FEN1 Antibody - LS-C19994 from LifeSpan Bioscience

Prices $295.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Anti-FEN1 Antibody - LS-C65983 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FEN1 Antibody - LS-C65982 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FEN1 Antibody (aa1-380) - LS-C83532 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-FEN1 Antibody (clone 2357C3a) - LS-C184782 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2357C3a

Anti-FEN1 Antibody (aa301-350) - LS-C80825 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.

Anti-FEN1 Antibody (N-Terminus) - LS-C43020 from LifeSpan Bioscience

Prices $520.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of human Fen1 in whole cell lysates of HeLa and 293T cells (5-50 ug/lane) by Western blotting with Rabbit anti-Human Fen1 (LS-C43020). Immunoprecipitation was with LS-C43020 (3 ug/mg lysate) or another anti-Fen1 polyclonal antibody, subsequent detection of immunoprecipitated Fen1 by Western blotting used LS-C43020.

Anti-FEN1 Antibody (clone 1E2) - LS-C197211 from LifeSpan Bioscience

Prices $355.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 1E2
FEN1 monoclonal antibody (M01A), clone 1E2 Western Blot analysis of FEN1 expression in HeLa NE.

Anti-FEN1 Antibody (C-Terminus) - LS-C203149 from LifeSpan Bioscience

Prices $245.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal
Western blot of cell lysates using 1 ug/ml Rabbit Anti-FEN-1 Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 45 kD. Observed Size: 45 kD.

Anti-FEN1 Antibody (N-Terminus) - LS-C145868 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FEN1 antibody LS-C145868 Western blot of 293T Cell lysate. Antibody concentration 1 ug/ml.

Anti-FEN1 Antibody - LS-C310355 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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