All lanes : Anti-FGD2 antibody [EPR15306] - N-terminal (ab185968) at 1/10000 dilutionLane 1 : Human tonsil lysateLane 2 : Human lymph node lysateLane 3 : Raji lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FGD2 with ab185968 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling FGD2 with ab185968 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunofluorescent analysis of acetone-fixed Raji cells labeling FGD2 with ab185968 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).