Anti-FGF 23 antibody
| Name | Anti-FGF 23 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56326 |
| Prices | $378.00 |
| Sizes | 200 µl |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: C-RHTRSAEDDSERD , corresponding to internal sequence amino acids 176-188 of Human FGF 23 Run BLAST with Run BLAST with |
| Description | Goat Polyclonal |
| Gene | FGF23 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-FGF 23 antibody (ab56326) at 0.5 µg/ml + Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer)
ICC/IF image of ab56326 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Phosphaturic mesenchymal tumor, nonphosphaturic variant, causing fatal pulmonary - Phosphaturic mesenchymal tumor, nonphosphaturic variant, causing fatal pulmonary
Uchihashi K, Nishijima-Matsunobu A, Matsuyama A, Yamasaki F, Tanabe T, Uemura T, Aragane N, Yakushiji M, Yamamoto M, Aoki S, Toda S. Hum Pathol. 2013 Nov;44(11):2614-8.
Autocrine/paracrine action of vitamin D on FGF23 expression in cultured rat - Autocrine/paracrine action of vitamin D on FGF23 expression in cultured rat
Tang WJ, Wang LF, Xu XY, Zhou Y, Jin WF, Wang HF, Gao J. Calcif Tissue Int. 2010 May;86(5):404-10.