All lanes : Anti-FGF 23 antibody (ab98000) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 4 : Bone (Human) Tissue Lysate - tumor tissue (ab29359)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.