Anti-FGF basic antibody
| Name | Anti-FGF basic antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8880 |
| Prices | $379.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | DB ICC/IF IHC-P WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Mammalian |
| Antigen | Synthetic peptide, corresponding to amino acids 1-23 of Rat FGF basic |
| Description | Rabbit Polyclonal |
| Gene | FGF2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab8880 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8880, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab8880 staining FGF basic in Mouse olfactory epithelium tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 1% blocking reagent from a tyramide signal amplification kit for 30 minutes at 23°C. The primary antibody was diluted 1/200 in the blocking reagent and incubated with the sample for 2 hours at 23°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/100. After secondary antibody incubation, the signal was amplified using a tyramide signal amplication kit with Alexa Fluor® 488 tyramide.See Abreview
Product References
Modulation of neural stem/progenitor cell proliferation during experimental - Modulation of neural stem/progenitor cell proliferation during experimental
Rotschafer JH, Hu S, Little M, Erickson M, Low WC, Cheeran MC. Neurobiol Dis. 2013 Oct;58:144-55.
ATP differentially upregulates fibroblast growth factor 2 and transforming growth - ATP differentially upregulates fibroblast growth factor 2 and transforming growth
Jia C, Cussen AR, Hegg CC. Neuroscience. 2011 Mar 17;177:335-46.