![Overlay histogram showing HEK293 cells stained with ab117640 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117640, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18617_ab117640-5-ab117640FC.jpg)
Overlay histogram showing HEK293 cells stained with ab117640 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117640, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Human Placenta, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117640 at 5µg/ml.
Immunofluorescence analysis of FGF1 expression on HeLa cells with ab117640 at 10µg/ml.
![All lanes : Anti-FGF1 antibody [2E12] (ab117640)Lane 1 : Transfected 293T cell line Lane 2 : Non-Transfected 293T cell line](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18620_FGF1-Primary-antibodies-ab117640-3.jpg)
All lanes : Anti-FGF1 antibody [2E12] (ab117640)Lane 1 : Transfected 293T cell line Lane 2 : Non-Transfected 293T cell line
The detection limit for ab117640 is approximately 10ng/ml as a capture antibody.