FGF1

Gene Symbol	FGF1
Entrez Gene	2246
Alt Symbol	AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1
Species	Human
Gene Type	protein-coding
Description	fibroblast growth factor 1 (acidic)
Other Description	beta-endothelial cell growth factor\|endothelial cell growth factor, alpha\|endothelial cell growth factor, beta\|fibroblast growth factor 1\|heparin-binding growth factor 1
Swissprots	B2R5T0 P05230 Q16588 P07502 D3DQF2
Accessions	AAA52446 AAA52638 AAS99352 CAA41788 CAH18501 CAH23443 EAW61881 EAW61882 EAW61884 EAW61885 P05230 AI283639 AK289762 BAF82451 AK312301 BAG35227 BC032697 AAH32697 BG706412 DA111414 DA126618 DA189432 DA228737 DA326831 DA388578 DA389088 DA575048 DA649213 DA660034 DB092576 DB220987 DC344309 DQ655917 L01485 AAA52425 L01487 AAA52447 M13361 AAA79245 M60515 AAA51672 M60516 AAA51673 M74028 S67291 AAB29057 S67292 AAB29058 S67294 AAB29059 X51943 CAA36206 X65778 CAA46661 X65779 CAA46662 XM_005268390 XP_005268447 XM_011537607 XP_011535909 XM_011537608 XP_011535910 NM_000800 NP_000791 NM_001144892 NP_001138364 NM_001144934 NP_001138406 NM_001144935 NP_001138407 NM_001257205 NP_001244134 NM_001257206 NP_001244135 NM_001257207 NP_001244136 NM_001257208 NP_001244137 NM_001257209 NP_001244138 NM_001257210 NP_001244139 NM_001257211 NP_001244140 NM_001257212 NP_001244141 NM_033136 NP_149127 NM_033137 NP_149128
Function	Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. {ECO:0000269\|PubMed:16597617, ECO:0000269\|PubMed:20145243, ECO:0000269\|PubMed:8663044}.
Subcellular Location	Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Note=Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. {ECO:0000250}.
Tissue Specificity	Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. {ECO:0000269\|PubMed:11964394, ECO:0000269\|PubMed:7504343}.
Top Pathways	Melanoma, MAPK signaling pathway, PI3K-Akt signaling pathway, Hippo signaling pathway, Rap1 signaling pathway

Acidic FGF (11H11) Rabbit mAb - 3139 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	11H11

Western blot analysis of recombinant human aFGF at various concentrations, using Acidic FGF (11H11) Rabbit mAb.

FGF-1 (B-3) - sc-55520 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

FGF-1 (B-3): sc-55520. Western blot analysis of human recombinant FGF-1.

FGF-1 (D-11) - sc-13123 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

FGF-1 (D-11): sc-13123. Western blot analysis of human recombinant FGF-1.

FGF-1 (C-18) - sc-1359 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Western blot analysis of human recombinant FGF-1. Antibodies tested include FGF-1 (C-18): sc-1359 (A) and FGF-1 (H-125): sc-7910 (B).

FGF-1 (C-19) - sc-1884 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

FGF-1 (C-19): sc-1884. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreatic tumor showing cytoplasmic staining.

FGF-1 (H-125) - sc-7910 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-FGF1 alpha antibody - ab169748 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling FGF1 alpha with ab169748 at 10µg/ml.

Anti-FGF1 alpha antibody [1F9] - ab119121 from Abcam

Prices	$464.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F9

Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Human Placenta tissue using ab119121 at (5 µg/ml).

Anti-FGF1 alpha antibody [4D2] - ab117863 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4D2

All lanes : Anti-FGF1 alpha antibody [4D2] (ab117863) at 1/5000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY FGF1 alpha cDNA Lysates/proteins at 5 µg per lane.

Anti-FGF1 antibody - ab9588 from Abcam

Prices	$388.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ICC/IF image of ab9588 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FGF1 antibody - ab54677 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

FGF1 antibody (ab54677) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

Anti-FGF1 antibody - ab126845 from Abcam

Prices	$372.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-FGF1 antibody - ab58311 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

FGF1 antibody (ab58311) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.

Anti-FGF1 antibody - ab90669 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-FGF1 antibody - ab18963 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-FGF1 antibody (ab18963) at 1 µg/ml + Spinal Cord (Rat) Tissue Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-FGF1 antibody - ab49996 from Abcam

Prices	$370.00
Sizes	200 µg
Host	Rabbit
Clonality	Polyclonal

Anti-FGF1 antibody - ab103577 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-FGF1 antibody (ab103577) at 1/500 dilution + Human placenta tissue lysate at 25 µg

Anti-FGF1 antibody [2E12] - ab117640 from Abcam

Prices	$387.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	2E12

Overlay histogram showing HEK293 cells stained with ab117640 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117640, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-FGF1 antibody (Biotin) - ab83163 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-FGF1 antibody [EP10920] - ab179455 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EP10920

All lanes : Anti-FGF1 antibody [EP10920] (ab179455) at 1/1000 dilutionLane 1 : Human glioma lysateLane 2 : rat kidney lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-FGF1 antibody [EPR1178] - ab183494 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR1178

Anti-FGF1 antibody [EPR1178] (ab183494) at 1/2000 dilution + Mouse kidney lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-FGF1 antibody [KT115] - ab179878 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	KT115

Anti-FGF1 antibody - N-terminal - ab75928 from Abcam

Prices	$378.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-FGF1 antibody - N-terminal (ab75928) at 1/50 dilutionLane 1 : Non transfected 293T cell lysateLane 2 : 293T cell lysate transiently transfected with the FGF1 geneLysates/proteins at 12.5 µg per lane.

Anti-FGF1 Antibody (clone 3A11G5) - LS-C203208 from LifeSpan Bioscience

Prices	$245.00
Sizes	40 µg
Host	Mouse
Clonality	Monoclonal
Clone	3A11G5

Anti-FGF1 / Acidic FGF Antibody (N-Terminus) - LS-C134973 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

FGF1 antibody LS-C134973 Western blot of Rat Kidney lysate.

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