Anti-FGFR2 antibody
| Name | Anti-FGFR2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58201 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 621-724 of Human FGFR2 |
| Description | Mouse Monoclonal |
| Gene | FGFR2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
FGFR2 antibody (ab58201) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach carcinoma tissue.
Western blot against tagged recombinant protein immunogen using ab58201 FGFR2 antibody at 1ug/ml.
ab40880 staining FGFR2 in Human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 0.5% BSA for 30 minutes at room temperature. The primary antibody was diluted 1/50 and incubated with the sample for 16 hours at 4°C. The secondary antibody was TRITC-conjugated Goat anti-Mouse polyclonal, diluted 1/300.See Abreview
![Overlay histogram showing HeLa cells stained with ab58201 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58201, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18820_FGFR2-Primary-antibodies-ab58201-42.jpg)
Overlay histogram showing HeLa cells stained with ab58201 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58201, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunohistochemical analysis of Human gastric adenocarcinoma, staining FGFR2 with ab58201 at 0.2 µg/ml.
Product References
FGF10 Signaling differences between type I pleuropulmonary blastoma and - FGF10 Signaling differences between type I pleuropulmonary blastoma and
Lezmi G, Verkarre V, Khen-Dunlop N, Vibhushan S, Hadchouel A, Rambaud C, Copin MC, Rittie JL, Benachi A, Fournet JC, Delacourt C. Orphanet J Rare Dis. 2013 Sep 3;8:130.
Multidimensional identification of tissue biomarkers of gastric cancer. - Multidimensional identification of tissue biomarkers of gastric cancer.
Guo T, Fan L, Ng WH, Zhu Y, Ho M, Wan WK, Lim KH, Ong WS, Lee SS, Huang S, Kon OL, Sze SK. J Proteome Res. 2012 Jun 1;11(6):3405-13.