Western blot analysis of extracts from ACHN cells, untreated or treated with Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min), and C2C12 cells, untreated or insulin-treated (100 nM, 30 min), using Phospho-Bad (Ser136) (D25H8) Rabbit mAb (upper) or Bad (D24A9) Rabbit mAb #9239 (lower).
Western blot analysis of extracts from COS-7 cells, treated with Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min) in the presence or absence of calf intestinal phosphatase (CIP) plus lambda-phosphatase, using Phospho-Bad (Ser136) (D25H8) Rabbit mAb (upper) or Bad (D24A9) Rabbit mAb #9239 (lower).
Western blot analysis of lysates containing Bad Control Proteins #9293, comprised of nonphosphorylated and phosphorylated Bad peptides, using Phospho-Bad (Ser136) (D25H8) Rabbit mAb.