BAD

Gene Symbol BAD
Entrez Gene 572
Alt Symbol BBC2, BCL2L8
Species Human
Gene Type protein-coding
Description BCL2-associated agonist of cell death
Other Description BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein|bcl-2-binding component 6|bcl-2-like protein 8|bcl-XL/Bcl-2-associated death promoter|bcl2 antagonist of cell death|bcl2-L-8|bcl2-associated agonist of cell death
Swissprots O14803 Q6FH21 Q92934
Accessions EAW74233 EAW74234 EAW74235 EAW74236 Q92934 AB451254 BAG70068 AB451378 BAG70192 AF021792 AAB72092 AF031523 AAB88124 AK023420 AK291863 BAF84552 AK303051 BAG64171 AK309150 AW014069 BC001901 AAH01901 BC095431 AAH95431 BE255791 BG435829 BG748336 BT006678 AAP35324 CR541935 CAG46733 CR541959 CAG46757 DQ895784 ABM86710 EU176363 ABW03814 U66879 AAB36516 NM_004322 NP_004313 NM_032989 NP_116784
Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. {ECO:0000250}.
Subcellular Location Mitochondrion outer membrane. Cytoplasm {ECO:0000250|UniProtKB:Q61337}. Note=Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm. {ECO:0000250|UniProtKB:Q61337}.
Tissue Specificity Expressed in a wide variety of tissues.
Top Pathways Apoptosis, Hepatitis C, cGMP-PKG signaling pathway, PI3K-Akt signaling pathway, Viral carcinogenesis

Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated) - 5193 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 7E11
Western blot analysis of extracts from OVCAR8 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated).

Phospho-Bad (Ser112) (40A9) Rabbit mAb - 5284 from Cell Signaling Technology

Prices $109.00, $307.00, $719.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 40A9
Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb (upper) or Bad Antibody #9292 (lower).

Phospho-Bad (Ser136) (D25H8) Rabbit mAb - 4366 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D25H8
Western blot analysis of extracts from ACHN cells, untreated or treated with Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min), and C2C12 cells, untreated or insulin-treated (100 nM, 30 min), using Phospho-Bad (Ser136) (D25H8) Rabbit mAb (upper) or Bad (D24A9) Rabbit mAb #9239 (lower).

Phospho-Bad (Ser112) (7E11) Mouse mAb - 9296 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 200 µl (40 western blots)
Host Mouse
Clonality Monoclonal
Clone 7E11
Western blot analysis of extracts from COS cells untreated or TPA-treated, using Phospho-Bad (Ser112) (7E11) Mouse mAb (upper) or Bad Antibody #9292 (lower).

Phospho-Bad (Ser136) Antibody - 9295 from Cell Signaling Technology

Prices $287.00
Sizes 200 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of GST-Bad, phosphorylated by CKII or PKA in vitro, using Phospho-Bad (Ser112) Antibody #9291 (top), Phospho-Bad (Ser136) Antibody (middle) or Bad Antibody #9292 (bottom).

Phospho-Bad (Ser155) Antibody - 9297 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of cell extracts from 293 cells transfected with Wild-type Bad, Bad (S112A/S136A), Bad (S155A) and treated with forskolin (30 µM), using Phospho-Bad (Ser155) Antibody (upper) and Bad Antibody #9292 (lower).

Phospho-Bad (Ser112) Antibody - 9291 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A), Bad (S112A/S136A) and treated with TPA or forskolin, using Phospho-Bad (Ser112) Antibody (upper) or Bad Antibody #9292 (lower).

Phospho-Bad (Ser136) (185D10) Rabbit mAb - 5286 from Cell Signaling Technology

Prices $109.00, $307.00
Sizes 20 µl (2 western blots), 200 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 185D10
Western blot analysis of extracts from control 293, GST-Bad transfected 293 and COS cells, untreated or TPA-treated, using Phospho-Bad (Ser136) (185D10) Rabbit mAb.

Bad (11E3) Rabbit mAb (IP Preferred) - 9268 from Cell Signaling Technology

Prices $256.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 11E3
Western blot analysis of extracts from OVCAR8 (human), Neuro2A (mouse) and H4IIE (rat) cell lines using Bad (11E3) Rabbit mAb (IP Preferred).

Bad (D24A9) Rabbit mAb - 9239 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D24A9
Western blot analysis of extracts from various cell lines using Bad (D24A9) Rabbit mAb.

Bad Antibody - 9292 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® Bad siRNA I (+), using Bad Antibody and p42 MAPK Antibody #9108. The Bad antibody confirms silencing of Bad expression, while the p42 MAPK antibody is used to control for loading and specificity of Bad siRNA.

Phospho-Bad (Ser112) (7E11) Mouse mAb - 5280 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 7E11

Phospho-Bad (Ser136) (5D8) Mouse mAb - 5282 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 5D8

Bad (C-7) - sc-8044 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Bad (C-7): sc-8044. Western blot analysis of Bad expression in HeLa whole cell lysate.

Bad (K-14) - sc-6541 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Bad (H-168) - sc-7869 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of human recombinant Bad fusion protein (A,B). Antibodies tested include Bad (H-168): sc-7869 (A) and Bad (N-19): sc-6542 (B).

Bad (K-17) - sc-942 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Bad (K-17): sc-942. Western blot analysis of Bad expression in whole cell lysates prepared from control (A) and serum-starved (B) NIH/3T3 cells.

Bad (N-19) - sc-6542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of human recombinant Bad fusion protein (A,B). Antibodies tested include Bad (H-168): sc-7869 (A) and Bad (N-19): sc-6542 (B).

p-Bad (Ser 112) - sc-7998 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Bad (Ser 112)-R: sc-7998-R. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A) and human Bad transfected: sc-170552 (B) 293T whole cell lysates.

p-Bad (C-10) - sc-166932 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase (sc-200312A) treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (C-10): sc-166932 (A,B,C,D) and Bad (H-168): sc-7869 (E,F,G,H).

p-Bad (Ser 136) - sc-7999 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Bad (Ser 136)-R: sc-7999-R. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A) and human Bad transfected: sc-170552 (B) 293T whole cell lysates.

p-Bad (F-6) - sc-271963 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-Bad (Ser 136) - sc-12969 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Bad (Ser 155) - sc-133356 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-Bad (Ser 155) - sc-101641 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Bad (Ser 155): sc-101641. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing cytoplasmic staining.
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