Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)]

• Supplier: Abcam
• Catalog: ab155960
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2281(3)
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FGFR3 (phospho Y724)
• Description: Rabbit Monoclonal
• Gene: FGFR3
• Conjugate: Unconjugated

Product images

All lanes : Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 1/5000 dilution (unpurified)Lane 1 : MCF-7 cell lysate (untreated)Lane 2 : MCF-7 cell lysate (treated with pervanadate)Lysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 20000 (purified)Lane 1 : MCF-7 cell lysate (untreated)Lane 2 : MCF-7 cell lysate (treated with pervanadate)Lysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells treated with pervanadate labelling FGFR3 (phospho Y724) with purified ab155960 at 1/80 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence analysis of untreated MCF-7 cells labelling FGFR3 (phospho Y724) with purified ab155960 at 1/80. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells treated with pervanadate labelling FGFR3 (phospho Y724) with unpurified ab155960 at 1/300 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence analysis of untreated MCF-7 cells labelling FGFR3 (phospho Y724) with unpurified ab155960 at 1/300. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

ab155960 (unpurified) at 1/15 immunoprecipitating FGFR3 (phospho Y724) in MCF-7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

ab155960 (purified) at 1/50 immunoprecipitating FGFR3 (phospho Y724) in MCF-7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.