All lanes : Anti-FH antibody [EPR11647(2)(B)] - N-terminal (ab184945) at 1/2000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : 293 cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Immunofluorescence analysis of, -20 °C acetone-fixed, HeLa cells labeling FH with ab184945 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
Flow cytometry analysis of 2% paraformaldehyde-fixed HeLa cells labeling FH with ab184945 at a 1/160 dilution (red) or negative control rabbit IgG (green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.