Immunofluorescent analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 5 min, labeling FH with ab184644 at 1/500 dilution (green). Hoechst 33342 staining (blue).
All lanes : Anti-FH antibody [GT7612] - C-terminal (ab184644) at 1/500 dilutionLane 1 : 293T lysateLane 2 : A431 lysateLane 3 : HeLa lysateLane 4 : HepG2 lysateLysates/proteins at 30 µg per lane.
Anti-FH antibody [GT7612] - C-terminal (ab184644) at 1/1000 dilution + mouse liver lysate/extract at 50 µg
Anti-FH antibody [GT7612] - C-terminal (ab184644) at 1/1000 dilution + rat liver lysate/extract at 50 µg