Anti-FHIT antibody
| Name | Anti-FHIT antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15287 |
| Prices | $370.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Rabbit Polyclonal |
| Gene | FHIT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab15287 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Immunohistochemical evaluation of p53, FHIT, and IGF2 gene expression in - Immunohistochemical evaluation of p53, FHIT, and IGF2 gene expression in
Chava S, Mohan V, Shetty PJ, Manolla ML, Vaidya S, Khan IA, Waseem GL, Boddala P, Ahuja YR, Hasan Q. Dis Esophagus. 2012 Jan;25(1):81-7.
Immunohistochemical assessment of Fhit protein expression in advanced gastric - Immunohistochemical assessment of Fhit protein expression in advanced gastric
Czyzewska J, Guzinska-Ustymowicz K, Pryczynicz A, Kemona A, Bandurski R. Folia Histochem Cytobiol. 2009;47(1):47-53.