FHIT

Gene Symbol FHIT
Entrez Gene 2272
Alt Symbol AP3Aase, FRA3B
Species Human
Gene Type protein-coding
Description fragile histidine triad
Other Description AP3A hydrolase|bis(5'-adenosyl)-triphosphatase|diadenosine 5',5'''-P1,P3-triphosphate hydrolase|dinucleosidetriphosphatase|tumor suppressor protein
Swissprots A2IAT0 P49789 A8K1A9 Q6IU12 A2IAS9 Q45QG9 A2IAT6
Accessions AAB52539 AAB53793 AAB84098 AAT37530 ABD93870 ABD93871 ABG02271 ABG02272 ABG02273 ABG02274 ABG02275 ABG81420 ABI15739 ABI15740 ABI15741 ABI15742 ABM65879 ABM66086 ABM66087 ABM66088 ABM66089 ABM66090 ABM66091 ABM66092 ABM66093 CAY55966 EAW65393 EAW65394 P49789 AK127931 AK289824 BAF82513 BC032336 AAH32336 BC057223 DA239143 DQ120721 AAZ23623 DQ891575 ABM82501 DQ894769 ABM85695 KJ534835 AHW56475 U46922 AAA99013 XM_011533481 XP_011531783 XM_011533482 XP_011531784 XM_011533483 XP_011531785 XM_011533484 XP_011531786 XM_011533485 XP_011531787 XM_011533486 XP_011531788 NM_001166243 NP_001159715 NM_002012 NP_002003
Function Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor. {ECO:0000269|PubMed:12574506, ECO:0000269|PubMed:15313915, ECO:0000269|PubMed:16407838, ECO:
Subcellular Location Cytoplasm. Mitochondrion. Nucleus.
Tissue Specificity Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.
Top Pathways Small cell lung cancer, Non-small cell lung cancer, Purine metabolism

Phospho-FHIT (Tyr114) Antibody - 4701 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

FHIT Antibody - 4704 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Fhit (C-7) - sc-271621 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fhit (C-7): sc-271621. Western blot analysis of Fhit expression in non-transfected: sc-117752 (A) and mouse Fhit transfected: sc-120253 (B) 293T whole cell lysates.

Fhit (G-4) - sc-390481 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fhit (G-4): sc-390481. Western blot analysis of Fhit expression in Caki-1 (A), ACHN (B) and A549 (C) whole cell lysates and human kidney tissue extract (D).

Fhit (C-20) - sc-8214 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Fhit (C-20): sc-8214. Western blot analysis of Fhit expression in non-transfected: sc-117752 (A) and human Fhit transfected: sc-114836 (B) 293T whole cell lysates.

Fhit (H-107) - sc-292538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Fhit (H-107): sc-292538. Western blot analysis of Fhit expression in non-transfected: sc-117752 (A) and human Fhit transfected: sc-114836 (B) 293T whole cell lysates and human kidney tissue extract (C).

Fhit (N-19) - sc-8215 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Fhit (N-19): sc-8215. Western blot analysis of Fhit expression in non-transfected: sc-117752 (A) and human Fhit transfected: sc-114836 (B) 293T whole cell lysates.

Anti-FHIT (phospho Y114) antibody - ab111557 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111557, at 1/50 dilution, staining FHIT in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-FHIT antibody - ab53074 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53074 at 1/50 dilution, with and without peptide immunogen.

Anti-FHIT antibody - ab15287 from Abcam

Prices $370.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab15287 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FHIT antibody - ab180806 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-FHIT antibody (ab180806) at 1/500 dilution + K562 cell lysate

Anti-FHIT antibody - ab58065 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-FHIT antibody [EPR10931] - ab170888 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10931
All lanes : Anti-FHIT antibody [EPR10931] (ab170888) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Rat brain lysateLane 3 : Rat kidney lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-FHIT antibody, prediluted - ab15288 from Abcam

Prices $370.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal

Anti-FHIT antibody - C-terminal - ab181004 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-FHIT antibody - C-terminal (ab181004) at 1 µgLane 2 : Anti-FHIT antibody - C-terminal (ab181004) at 2 µgLane 1 : HeLa cell lysateLane 2 : HeLa cell lysateLysates/proteins at 15 µg per lane.

Anti-FHIT Antibody - LS-C66023 from LifeSpan Bioscience

Prices $530.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-FHIT Antibody - LS-C189351 from LifeSpan Bioscience

Prices $485.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-FHIT Antibody - LS-C50630 from LifeSpan Bioscience

Prices $440.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
human breast stained with anti-FHIT antibody

Anti-FHIT Antibody (C-Terminus) - LS-C193971 from LifeSpan Bioscience

Prices $905.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FHIT Antibody (aa80-129) - LS-C291259 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FHIT Antibody (phospho-Tyr114) - LS-C198960 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human colon carcinoma, using FHIT (Phospho-Tyr114) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-FHIT Antibody (C-Terminus) - LS-C116412 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FHIT antibody LS-C116412 Western blot of Fetal Kidney lysate.

Anti-FHIT Antibody - LS-C192457 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of FHIT pAb in extracts from K562 cells.

Anti-FHIT Antibody (clone 1C3) - LS-C197220 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C3
Detection limit for recombinant GST tagged FHIT is approximately 0.1 ng/ml as a capture antibody.

Anti-FHIT Antibody (C-Terminus) - LS-C345642 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of FHIT Antibody with human MCF7 Whole Cell lysate.
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