Anti-FHL1 antibody
| Name | Anti-FHL1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58067 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: DGHHCCLKCF DKFCANTCVE CRKPIGADSK EVHYKNRFWH DTCFRCAKCL HPLANETFVA KDNKILCNKC TTREDSPKCK GCFKAIVAGD QNVEYKGT, corresponding to amino acids 23-121 of Human FHL1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | FHL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
FHL1 antibody (ab58067) at 1ug/lane + Raw cell lysate at 25ug/lane.
![Overlay histogram showing SH-SY5Y cells stained with ab58067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58067, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18997_FHL1-Primary-antibodies-ab58067-1.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab58067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58067, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
5'RNA-Seq identifies Fhl1 as a genetic modifier in cardiomyopathy. - 5'RNA-Seq identifies Fhl1 as a genetic modifier in cardiomyopathy.
Christodoulou DC, Wakimoto H, Onoue K, Eminaga S, Gorham JM, DePalma SR, Herman DS, Teekakirikul P, Conner DA, McKean DM, Domenighetti AA, Aboukhalil A, Chang S, Srivastava G, McDonough B, De Jager PL, Chen J, Bulyk ML, Muehlschlegel JD, Seidman CE, Seidman JG. J Clin Invest. 2014 Mar;124(3):1364-70.
Loss of FHL1 induces an age-dependent skeletal muscle myopathy associated with - Loss of FHL1 induces an age-dependent skeletal muscle myopathy associated with
Domenighetti AA, Chu PH, Wu T, Sheikh F, Gokhin DS, Guo LT, Cui Z, Peter AK, Christodoulou DC, Parfenov MG, Gorham JM, Li DY, Banerjee I, Lai X, Witzmann FA, Seidman CE, Seidman JG, Gomes AV, Shelton GD, Lieber RL, Chen J. Hum Mol Genet. 2014 Jan 1;23(1):209-25.
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Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy. - Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy.
Gueneau L, Bertrand AT, Jais JP, Salih MA, Stojkovic T, Wehnert M, Hoeltzenbein M, Spuler S, Saitoh S, Verschueren A, Tranchant C, Beuvin M, Lacene E, Romero NB, Heath S, Zelenika D, Voit T, Eymard B, Ben Yaou R, Bonne G. Am J Hum Genet. 2009 Sep;85(3):338-53.