All lanes : Anti-FHL1 antibody [1D6] (ab119043) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY FHL1 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-FHL1 antibody [1D6] (ab119043) at 1/1000 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab119043, at 1/100 dilution staining FHL1 in HEK293T cells transfected with either pCMV6-ENTRY FHL1 overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
ab119043 at 1/100 dilution staining FHL1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).