Anti-Fibroblast activation protein, alpha antibody
| Name | Anti-Fibroblast activation protein, alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53066 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Dog, Human, Monkey |
| Antigen | A synthetic peptide derived from human Fibroblast activation protein, alpha |
| Description | Rabbit Polyclonal |
| Gene | FAP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Fibroblast activation protein, alpha antibody (ab53066) at 1/500 dilutionLane 1 : LOVO cell extractLane 2 : LOVO cell extract with immunising peptideObserved band size : 90 kDa (why is the actual band size different from the predicted?)
ab53066 at 1/50 dilution staining Fibroblast activation protein, alpha in human ductal breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
ab53066 staining Fibroblast activation protein, alpha in Dog cancerous tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris/EDTA (pH 9.0) buffer. Samples were incubated with undiluted primary antibody (1/100 in antibody diluent) for 30 minutes at 21°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
ab53066 staining Fibroblast activation protein alpha in Monkey spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with Tris/EDTA (pH9). Samples were incubated with primary antibody (1/100 in a commercial diluent) for 30 minutes at 21°C. An undiluted HRP-conjugated Goat polyclonal was used as the secondary antibody.See Abreview
Immunofluorescence analysis of murine lung fibroblasts, staining Fibroblast activation protein with ab53066. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/100 in TBS) for 1 hour at 20°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. See Abreview
Immunofluorescence analysis of Human cancer-associated fibroblasts, staining Fibroblast activation protein, alpha with ab53066.
Product References
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Guan J, Zhang H, Wen Z, Gu Y, Cheng Y, Sun Y, Zhang T, Jia C, Lu Z, Chen J. Cancer Lett. 2014 Apr 1;345(1):132-9.
beta-Catenin-regulated myeloid cell adhesion and migration determine wound - beta-Catenin-regulated myeloid cell adhesion and migration determine wound
Amini-Nik S, Cambridge E, Yu W, Guo A, Whetstone H, Nadesan P, Poon R, Hinz B, Alman BA. J Clin Invest. 2014 Jun;124(6):2599-610.
Biological characteristics and genetic heterogeneity between carcinoma-associated - Biological characteristics and genetic heterogeneity between carcinoma-associated
Peng Q, Zhao L, Hou Y, Sun Y, Wang L, Luo H, Peng H, Liu M. PLoS One. 2013;8(4):e60321.
Virus-induced hepatocellular carcinomas cause antigen-specific local tolerance. - Virus-induced hepatocellular carcinomas cause antigen-specific local tolerance.
Willimsky G, Schmidt K, Loddenkemper C, Gellermann J, Blankenstein T. J Clin Invest. 2013 Mar;123(3):1032-43.
Deep vascular imaging in wounds by two-photon fluorescence microscopy. - Deep vascular imaging in wounds by two-photon fluorescence microscopy.
Yanez CO, Morales AR, Yue X, Urakami T, Komatsu M, Jarvinen TA, Belfield KD. PLoS One. 2013 Jul 2;8(7):e67559.
Fibroblast-derived dermal matrix drives development of aggressive cutaneous - Fibroblast-derived dermal matrix drives development of aggressive cutaneous
Ng YZ, Pourreyron C, Salas-Alanis JC, Dayal JH, Cepeda-Valdes R, Yan W, Wright S, Chen M, Fine JD, Hogg FJ, McGrath JA, Murrell DF, Leigh IM, Lane EB, South AP. Cancer Res. 2012 Jul 15;72(14):3522-34.
Cancer-associated fibroblasts from human NSCLC survive ablative doses of - Cancer-associated fibroblasts from human NSCLC survive ablative doses of
Hellevik T, Pettersen I, Berg V, Winberg JO, Moe BT, Bartnes K, Paulssen RH, Busund LT, Bremnes R, Chalmers A, Martinez-Zubiaurre I. Radiat Oncol. 2012 Apr 13;7:59.
Peptides from Lactobacillus hydrolysates of bovine milk caseins inhibit - Peptides from Lactobacillus hydrolysates of bovine milk caseins inhibit
Juillerat-Jeanneret L, Robert MC, Juillerat MA. J Agric Food Chem. 2011 Jan 12;59(1):370-7.
Characterization of phosphoglycerate kinase-1 expression of stromal cells derived - Characterization of phosphoglycerate kinase-1 expression of stromal cells derived
Wang J, Ying G, Wang J, Jung Y, Lu J, Zhu J, Pienta KJ, Taichman RS. Cancer Res. 2010 Jan 15;70(2):471-80.
Vascular endothelial growth factor secreted by activated stroma enhances - Vascular endothelial growth factor secreted by activated stroma enhances
Pinto MP, Badtke MM, Dudevoir ML, Harrell JC, Jacobsen BM, Horwitz KB. Cancer Res. 2010 Apr 1;70(7):2655-64.