Anti-Fibroblast activation protein, alpha antibody

• Supplier: Abcam
• Catalog: ab28244
• Prices: $384.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P FC WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide of Human Fibroblast activation protein, alpha, corresponding to the stalk region
• Description: Rabbit Polyclonal
• Gene: FAP
• Conjugate: Unconjugated

Product images

All lanes : Anti-Fibroblast activation protein, alpha antibody (ab28244) at 1/1000 dilutionLane 1 : Recombinant Human FAPalpha at 0.08 µgLane 2 : Recombinant Human FAPalpha at 0.04 µgLane 3 : Recombinant Human FAPalpha at 0.02 µgLane 4 : Recombinant Human FAPalpha at 0.01 µgLane 5 : Recombinant Human FAPalpha at 0.005 µg

ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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