Anti-Fibulin 5 antibody [1G6A4]
| Name | Anti-Fibulin 5 antibody [1G6A4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab66339 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1G6A4 |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant His tagged fragment, corresponding to amino acids 242-448 of Human Fibulin 5 |
| Description | Mouse Monoclonal |
| Gene | FBLN5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/2000 dilution + Human vascular smooth muscle whole cell lysate at 20 µgSecondaryHRP-conjugated Rabbit anti-mouse IgG polyclonal at 1/2000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/19275_ab66339-210121-ab66339wb.jpg)
Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/2000 dilution + Human vascular smooth muscle whole cell lysate at 20 µgSecondaryHRP-conjugated Rabbit anti-mouse IgG polyclonal at 1/2000 dilutionPerformed under reducing conditions.
![Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/500 dilution + Immunogen (recombinant truncated protein) at 1 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/19276_fb1.jpg)
Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/500 dilution + Immunogen (recombinant truncated protein) at 1 µg
Immunohistochemical analysis of paraffin embedded human colon cancer tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
ICC/IF image of ab66339 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66339, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Elastin development-associated extracellular matrix constituents of subepithelial - Elastin development-associated extracellular matrix constituents of subepithelial
Perez-Rico C, Pascual G, Sotomayor S, Asunsolo A, Cifuentes A, Garcia-Honduvilla N, Bujan J. Invest Ophthalmol Vis Sci. 2014 Sep 2;55(10):6309-18.
Fibulin-5 expression in the human placenta. - Fibulin-5 expression in the human placenta.
Gauster M, Berghold VM, Moser G, Orendi K, Siwetz M, Huppertz B. Histochem Cell Biol. 2011 Feb;135(2):203-13.
Fibulin-5 mutations link inherited neuropathies, age-related macular degeneration - Fibulin-5 mutations link inherited neuropathies, age-related macular degeneration
Auer-Grumbach M, Weger M, Fink-Puches R, Papic L, Frohlich E, Auer-Grumbach P, El Shabrawi-Caelen L, Schabhuttl M, Windpassinger C, Senderek J, Budka H, Trajanoski S, Janecke AR, Haas A, Metze D, Pieber TR, Guelly C. Brain. 2011 Jun;134(Pt 6):1839-52.