Anti-Filaggrin antibody
| Name | Anti-Filaggrin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab81468 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide derived from the N terminal domain of human Filaggrin |
| Description | Rabbit Polyclonal |
| Gene | FLG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Targeting epidermal lipids for treatment of Mendelian disorders of cornification. - Targeting epidermal lipids for treatment of Mendelian disorders of cornification.
Kiritsi D, Schauer F, Wolfle U, Valari M, Bruckner-Tuderman L, Has C, Happle R. Orphanet J Rare Dis. 2014 Mar 7;9:33.
siRNA silencing of proteasome maturation protein (POMP) activates the unfolded - siRNA silencing of proteasome maturation protein (POMP) activates the unfolded
Dahlqvist J, Torma H, Badhai J, Dahl N. PLoS One. 2012;7(1):e29471.