Anti-Filaggrin antibody [FLG01]
| Name | Anti-Filaggrin antibody [FLG01] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3137 |
| Prices | $382.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | FLG01 |
| Applications | IHC-F FC IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein |
| Description | Mouse Monoclonal |
| Gene | FLG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing A431 cells stained with ab3137 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3137, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/19310_Filaggrin-Primary-antibodies-ab3137-1.jpg)
Overlay histogram showing A431 cells stained with ab3137 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3137, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab3137 staining Filaggrin in Human skin tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde, blocked with 1% BSA for 30 minutes at 25°C and permeabilized with 0.2% Triton X-100. Samples were incubated with primary antibody (1/200 in diluent) for 1 hour. An AlexaFluor®594-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. See Abreview
Immunohistochemical analysis of cultured Human keratinocytes isolated from neonatal foreskin, staining Filaggrin with ab3137. Antigen retrieval was performed via heat mediation in citrate buffer, blocked in 3% BSA for 20 min, before incubating with primary antibody overnight at 4°C.
Product References
Highly rapid and efficient conversion of human fibroblasts to keratinocyte-like - Highly rapid and efficient conversion of human fibroblasts to keratinocyte-like
Chen Y, Mistry DS, Sen GL. J Invest Dermatol. 2014 Feb;134(2):335-44.
Effects of niacin restriction on sirtuin and PARP responses to photodamage in - Effects of niacin restriction on sirtuin and PARP responses to photodamage in
Benavente CA, Schnell SA, Jacobson EL. PLoS One. 2012;7(7):e42276.
Characterization of a unique technique for culturing primary adult human - Characterization of a unique technique for culturing primary adult human
Marcelo CL, Peramo A, Ambati A, Feinberg SE. BMC Dermatol. 2012 Jun 24;12:8.