Anti-Filaggrin antibody [SPM181]
| Name | Anti-Filaggrin antibody [SPM181] |
|---|---|
| Supplier | Abcam |
| Catalog | ab17808 |
| Prices | $392.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | SPM181 |
| Applications | FC IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | FLG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab17808 staining Filaggrin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.025% serum free protein block for 5 minutes at 25°C; antigen retrieval was by heat mediation with a TRIS/EDTA buffer (pH9). Samples were incubated with primary antibody (1/50 in serum free antibody diluent) for 30 minutes at 25°C. An undiluted HRP-conjugated Goat polymer was used as the secondary antibody.See Abreview
ab17808 staining Filaggrin in Human skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a TRIS/EDTA buffer (pH8.2). Samples were incubated with primary antibody (1/200 in antibody diluent) for 32 minutes at 37°C. A rabbit anti-mouse monoclonal (1/500) was used as the secondary antibody.See Abreview
![Overlay histogram showing A431 cells stained with ab17808 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17808, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879x) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/19315_Filaggrin-Primary-antibodies-ab17808-8.jpg)
Overlay histogram showing A431 cells stained with ab17808 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17808, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879x) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
The CD44+ ALDH+ population of human keratinocytes is enriched for epidermal stem - The CD44+ ALDH+ population of human keratinocytes is enriched for epidermal stem
Szabo AZ, Fong S, Yue L, Zhang K, Strachan LR, Scalapino K, Mancianti ML, Ghadially R. Stem Cells. 2013 Apr;31(4):786-99.
Expression microarray analysis identifies novel epithelial-derived protein - Expression microarray analysis identifies novel epithelial-derived protein
Matoso A, Mukkada VA, Lu S, Monahan R, Cleveland K, Noble L, Mangray S, Resnick MB. Mod Pathol. 2013 May;26(5):665-76.
Wharton's jelly stem cells: a novel cell source for oral mucosa and skin - Wharton's jelly stem cells: a novel cell source for oral mucosa and skin
Garzon I, Miyake J, Gonzalez-Andrades M, Carmona R, Carda C, Sanchez-Quevedo Mdel C, Campos A, Alaminos M. Stem Cells Transl Med. 2013 Aug;2(8):625-32.
Optimal differentiation of in vitro keratinocytes requires multifactorial - Optimal differentiation of in vitro keratinocytes requires multifactorial
Borowiec AS, Delcourt P, Dewailly E, Bidaux G. PLoS One. 2013 Oct 7;8(10):e77507.
Novel filaggrin mutation but no other loss-of-function variants found in - Novel filaggrin mutation but no other loss-of-function variants found in
Winge MC, Bilcha KD, Lieden A, Shibeshi D, Sandilands A, Wahlgren CF, McLean WH, Nordenskjold M, Bradley M. Br J Dermatol. 2011 Nov;165(5):1074-80.
Filaggrin expression in oral, nasal, and esophageal mucosa. - Filaggrin expression in oral, nasal, and esophageal mucosa.
De Benedetto A, Qualia CM, Baroody FM, Beck LA. J Invest Dermatol. 2008 Jun;128(6):1594-7.