Anti-Filamin A antibody
| Name | Anti-Filamin A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab11074 |
| Prices | $398.00 |
| Sizes | 200 µl |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ICC/IF ICC/IF WB ICC/IF FC |
| Species Reactivities | Chicken, Human, Xenopus, Zebrafish |
| Antigen | Full length native protein (purified) (Chicken) |
| Description | Goat Polyclonal |
| Gene | FLNA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab11074 staining Filamin A in Human platelet cells by Flow cytometry.Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated donkey anti-goat IgG (H+L) at a 1/500 dilution. US: Unstained, Red Peak; IgG Ms: IgG Mouse (Isotype Control), Blue Peak; Filamin: Filamin A, Green peak.See Abreview
ab11074 at a 1/2000 dilution staining Filamin A in XTC embryonic polyclonal fibroblasts by Immunocytochemistry/ Immunofluorescence.Cells were fixed with formaldehyde, permeabilized using Triton X-100 and blocked with 1% BSA. The secondary used was a Texas Red conjugated anti-goat, 1/200 dilution.
Anti-Filamin A antibody (ab11074) at 1/6000 dilution + Human platelet lysate at 20 µgSecondaryRabbit anti-Goat HRP at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling - The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling
Samwer M, Dehne HJ, Spira F, Kollmar M, Gerlich DW, Urlaub H, Gorlich D. EMBO J. 2013 Jul 3;32(13):1886-902.
Androgen-induced cell migration: role of androgen receptor/filamin A association. - Androgen-induced cell migration: role of androgen receptor/filamin A association.
Castoria G, D'Amato L, Ciociola A, Giovannelli P, Giraldi T, Sepe L, Paolella G, Barone MV, Migliaccio A, Auricchio F. PLoS One. 2011 Feb 16;6(2):e17218.
PACSIN2 regulates cell adhesion during gastrulation in Xenopus laevis. - PACSIN2 regulates cell adhesion during gastrulation in Xenopus laevis.
Cousin H, Desimone DW, Alfandari D. Dev Biol. 2008 Jul 1;319(1):86-99.
Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A - Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A
Boraldi F, Annovi G, Carraro F, Naldini A, Tiozzo R, Sommer P, Quaglino D. Biochim Biophys Acta. 2007 Nov;1774(11):1402-13. Epub 2007 Aug 22.