Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A), Bad (S112A/S136A) and treated with TPA or forskolin, using Phospho-Bad (Ser112) Antibody (upper) or Bad Antibody #9292 (lower).
Western blot analysis of Bad fusion protein phosphorylated by CKII or PKA in vitro, using Phospho-Bad (Ser112) Antibody (upper) or Bad Antibody #9292 (lower).
Flow cytometric analysis of COS cells, untreated (blue) or Calyculin A/TPA-treated (green), usingPhospho-Bad (Ser112) Antibody compared to Rabbit (DA1E) mAb IgG Isotype Control #3900 (red).