Anti-FKBP12 antibody
| Name | Anti-FKBP12 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58072 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Hamster, Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-109 of Human FKBP12 |
| Description | Mouse Monoclonal |
| Gene | FKBP1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab58072 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58072, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab58072 staining in human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58072, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Prophase pathway-dependent removal of cohesin from human chromosomes requires - Prophase pathway-dependent removal of cohesin from human chromosomes requires
Buheitel J, Stemmann O. EMBO J. 2013 Mar 6;32(5):666-76.
Impact of hypoxia, simulated ischemia and reperfusion in HL-1 cells on the - Impact of hypoxia, simulated ischemia and reperfusion in HL-1 cells on the
Astrom-Olsson K, Li L, Olofsson CS, Boren J, Ohlin H, Grip L. Biochem Biophys Res Commun. 2012 Jun 15;422(4):732-8. doi: