All lanes : Anti-FKBP12 antibody (ab24373) at 1 µg/mlLane 1 : Human Brain Tissue LysateLane 2 : Mouse Brain Tissue LysateLane 3 : Rat Brain Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
ab24373 staining FKBP12 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde before heat mediated antigen retrieval and then blocked for 45 minutes at 20°C (5 minutes of peroxidase block and 10 minutes of protein block). The primary antibody was diluted, 1/2000 in PBS and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. See Abreview
Anti-FKBP12 antibody (ab24373) at 1 µg/ml + Human FKBP12 full length protein (ab56524) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 2 minutes