Anti-FLI1 antibody [EPR4646]

• Supplier: Abcam
• Catalog: ab133485
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4646
• Applications: IHC-P WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Blocking Peptide: FLI1 peptide
• Description: Rabbit Monoclonal
• Gene: FLI1
• Conjugate: Unconjugated

Product images

Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µgSecondaryHRP goat anti-rabbit at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical staining of paraffin embedded rat spleen with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Anti-FLI1 antibody [EPR4646] (ab133485) at 1/200 dilution (unpurified) + Jurkat cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)Lane 1 : U937 cell lysateLane 2 : Jurkat cell lysateLane 3 : Raw264.7 cell lysateLane 4 : HL-60 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)Lane 1 : FLI1 recombinant proteinLane 2 : ERG recombinant proteinLysates/proteins at 0.01 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical staining of paraffin embedded rat spleen with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.