FLI1

Gene Symbol FLI1
Entrez Gene 2313
Alt Symbol EWSR2, SIC-1
Species Human
Gene Type protein-coding
Description Fli-1 proto-oncogene, ETS transcription factor
Other Description Ewing sarcoma breakpoint region 2|Friend leukemia integration 1 transcription factor|Friend leukemia virus integration 1|transcription factor ERGB
Swissprots Q14319 Q01543 B4DFV4 G3V183 B4DTC6 B2R8H2 Q9UE07 Q92480
Accessions AAA76854 AAG33027 BAA07463 BAA32805 BAA32806 CAA76731 CDL93502 EAW67715 EAW67716 EAW67717 EAW67718 EAW67719 EAW67720 Q01543 AF147318 AI354789 AK294279 BAG57565 AK300153 BAG61938 AK313370 BAG36169 AY029368 AAK50443 BC001670 AAH01670 BC010115 AAH10115 BX647094 DC381599 DQ892582 ABM83508 DQ896858 ABM87857 M93255 AAA58479 M98833 AAA35812 S45205 AAB23637 S64709 X67001 CAA47399 XM_011542701 XP_011541003 XM_011542702 XP_011541004 NM_001167681 NP_001161153 NM_001271010 NP_001257939 NM_001271012 NP_001257941 NM_002017 NP_002008
Function Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Subcellular Location Nucleus.
Top Pathways Transcriptional misregulation in cancer

FLI1 Antibody - 12225 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using FLI1 Antibody.

Fli-1 (F-12) - sc-365294 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fli-1 (F-12): sc-365294. Western blot analysis of Fli-1 expression in U-937 (A) and RAW 264.7 (B) whole cell lysates.

Fli-1 (7.3) - sc-53826 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fli-1 (7.3): sc-53826. Western blot analysis of Fli-1 expression in U-937 whole cell lysate.

Fli-1 (C-19) - sc-356 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Fli-1 (C-19): sc-356. Western blot analysis of Fli-1 expression in RAW 264.7 (A), HL-60 (B), U-937 (C) and BJAB (D) whole cell lysates.

Fli-1 (H-60) - sc-22808 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Fli-1 (H-60): sc-22808. Western blot analysis of Fli-1 expression in BJAB (A) and MEG-01 (B) whole cell lysates.

Fli-1 (23D10) - sc-134223 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fli-1 (23D10): sc-134223. Western blot analysis of Fli-1 expression in HEL 92.1.7 whole cell lysate. Kindly provided by Dr. You Jun li of Ben-Davids Laboratory, Sunnybrook Health Sciences Centre.

Anti-FLI1 antibody - ab53077 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53077 at 1/50 dilution, with and without immunizing peptide.

Anti-FLI1 antibody - ab153909 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FLI1 antibody (ab153909) at 1/1000 dilutionLane 1 : Raji whole cell lysateLane 2 : THP1 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-FLI1 antibody - ab90583 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FLI1 antibody (ab90583) at 1 µg/mlLane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FLI1 antibody - ab49045 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lane 1 :  MARKERLane 2 :  Anti-FLI1 antibody (ab49045) at 1.25 µg/mlLane 1 : As aboveLane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.SecondaryLane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-FLI1 antibody - ab90705 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-FLI1 antibody (ab90705) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-FLI1 antibody - ab11851 from Abcam

Prices $370.00
Sizes 125 µg
Host Sheep
Clonality Polyclonal

Anti-FLI1 antibody - ab55012 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab55012 FLI1 antibody at 1ug/ml.

Anti-FLI1 antibody [BV4], prediluted - ab56178 from Abcam

Prices $376.00
Sizes 6 ml
Host Rabbit
Clonality Monoclonal
Clone BV4
ab56178 staining FLI1 in formalin-fixed, paraffin-embedded Ewing's sarcoma tissue by Immunohistochemistry.

Anti-FLI1 antibody [EPR4645] - ab124791 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4645
All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilutionLane 1 : Raw264.7 cell lysateLane 2 : U937 cell lysateLane 3 : Jurkat cell lysateLane 4 : K562 cell lysateLane 5 : C6 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-FLI1 antibody [EPR4646] - ab133485 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4646
Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µgSecondaryHRP goat anti-rabbit at 1/1000 dilution

Anti-FLI1 antibody [EPR4646] (Alexa Fluor® 488) - ab196371 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4646
ab196371 staining FLI1 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196371 at a working dilution of 1 in 50 (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FLI1 antibody [EPR4646] (Alexa Fluor® 647) - ab196146 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4646
ab196146 staining FLI-1 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196146 at 1/50 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FLI1 antibody [EPR4646] (DyLight® 488) - ab139914 from Abcam

Prices $337.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4646
Immunofluorescent analysis of HT29 cells labelling FLI1 with ab139914 at 1/100 dilution (green) and DAPI staining (blue).

Anti-FLI1 antibody, prediluted - ab15290 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15290 staining FLI1 in angiosarcoma by Immunohistochemistry (FFPE-sections).

Anti-FLI1 antibody - C-terminal - ab180902 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 :  Anti-FLI1 antibody - C-terminal (ab180902) at 0.5 µg/mlLane 2 :  Anti-FLI1 antibody - C-terminal (ab180902) at 1 µg/mlLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 15 µg per lane.

Anti-FLI1 antibody - ChIP Grade - ab15289 from Abcam

Prices $401.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab15289, at 1/50 dilution, staining FLI1 in angiosarcoma by Immunohistochemistry (FFPE-sections).

Anti-FLI1 Antibody - LS-C26562 from LifeSpan Bioscience

Prices $330.00
Sizes 250 µg
Host Sheep
Clonality Polyclonal

Anti-FLI1 Antibody (C-Terminus) - LS-C66176 from LifeSpan Bioscience

Prices $485.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-FLI1 Antibody - LS-C88804 from LifeSpan Bioscience

Prices $290.00, $460.00, $770.00
Sizes 100 µl, 500 µl, 1 ml
Host Rabbit
Clonality Polyclonal
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