Anti-FLI1 antibody - ChIP Grade
| Name | Anti-FLI1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab15289 |
| Prices | $401.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP WB IP ChIPseq IHC-P |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) |
| Description | Rabbit Polyclonal |
| Gene | FLI1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab15289, at 1/50 dilution, staining FLI1 in angiosarcoma by Immunohistochemistry (FFPE-sections).
Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 0.4% and 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.See Abreview
All lanes : Anti-FLI1 antibody - ChIP Grade (ab15289) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Thrombocytopenia-associated mutations in the ANKRD26 regulatory region induce - Thrombocytopenia-associated mutations in the ANKRD26 regulatory region induce
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Key regulators control distinct transcriptional programmes in blood progenitor - Key regulators control distinct transcriptional programmes in blood progenitor
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Molecular dissection of the mechanism by which EWS/FLI expression compromises - Molecular dissection of the mechanism by which EWS/FLI expression compromises
Chaturvedi A, Hoffman LM, Jensen CC, Lin YC, Grossmann AH, Randall RL, Lessnick SL, Welm AL, Beckerle MC. Mol Biol Cell. 2014 Sep 15;25(18):2695-709.
NLS-tagging: an alternative strategy to tag nuclear proteins. - NLS-tagging: an alternative strategy to tag nuclear proteins.
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BCL11B is up-regulated by EWS/FLI and contributes to the transformed phenotype in - BCL11B is up-regulated by EWS/FLI and contributes to the transformed phenotype in
Wiles ET, Lui-Sargent B, Bell R, Lessnick SL. PLoS One. 2013;8(3):e59369.
Genome-wide DNA methylation profiles in hematopoietic stem and progenitor cells - Genome-wide DNA methylation profiles in hematopoietic stem and progenitor cells
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A Runx1-Smad6 rheostat controls Runx1 activity during embryonic hematopoiesis. - A Runx1-Smad6 rheostat controls Runx1 activity during embryonic hematopoiesis.
Knezevic K, Bee T, Wilson NK, Janes ME, Kinston S, Polderdijk S, Kolb-Kokocinski A, Ottersbach K, Pencovich N, Groner Y, de Bruijn M, Gottgens B, Pimanda JE. Mol Cell Biol. 2011 Jul;31(14):2817-26.
Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide - Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide
Wilson NK, Foster SD, Wang X, Knezevic K, Schutte J, Kaimakis P, Chilarska PM, Kinston S, Ouwehand WH, Dzierzak E, Pimanda JE, de Bruijn MF, Gottgens B. Cell Stem Cell. 2010 Oct 8;7(4):532-44.
Spi-1 and Fli-1 directly activate common target genes involved in ribosome - Spi-1 and Fli-1 directly activate common target genes involved in ribosome
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O-GlcNAcylation is involved in the transcriptional activity of EWS-FLI1 in - O-GlcNAcylation is involved in the transcriptional activity of EWS-FLI1 in
Bachmaier R, Aryee DN, Jug G, Kauer M, Kreppel M, Lee KA, Kovar H. Oncogene. 2009 Mar 5;28(9):1280-4.