All lanes : Anti-FLIP antibody (ab84283) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab84283 (1/200) staining FLIP in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised using 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.See Abreview