CFLAR

Gene Symbol CFLAR
Entrez Gene 8837
Alt Symbol CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS
Species Human
Gene Type protein-coding
Description CASP8 and FADD-like apoptosis regulator
Other Description FADD-like antiapoptotic molecule 1|MACH-related inducer of toxicity|caspase homolog|caspase-eight-related protein|caspase-like apoptosis regulatory protein|caspase-related inducer of apoptosis|cellular FLICE-like inhibitory protein|inhibitor of FLICE|usurpin beta
Swissprots O43620 B4DJE0 Q54AF1 O60459 O43618 O14673 O14675 Q9UEW1 O14674 O15356 O43619 O15519 O15510 O15137 Q96TE4 O15138 B7Z9F9 Q53TS6 O60458
Accessions AAY24290 BAB32551 BAB32552 CCA94581 EAW70235 EAW70236 EAW70237 EAW70238 EAW70239 EAW70240 EAW70241 EAW70242 EAW70243 EAW70244 EAW70245 O15519 AB209600 BAD92837 AF005774 AAC15825 AF005775 AAC15826 AF009616 AAB70909 AF009617 AAB70910 AF009618 AAB70911 AF009619 AAB70912 AF010127 AAB64110 AF015450 AAC16439 AF015451 AAC16440 AF015452 AAC16441 AF041458 AAB99790 AF041459 AAB99791 AF041460 AAB99792 AF041461 AAB99793 AF041462 AAB99794 AK124664 AK125394 AK289913 BAF82602 AK296036 BAG58802 AK297387 BAG59828 AK304585 BAG65373 AK308767 AK315208 BAG37645 AK315924 BAH14295 AL365413 BC001602 AAH01602 BM969271 BT006751 AAP35397 BX457155 CB148618 CD699251 DC321068 DC375144 DQ890592 ABM92209 DQ893760 ABM84686 U85059 AAB82648 U97074 AAC51622 U97075 AAC51623 Y14039 CAA74366 Y14040 CAA74367 XM_005246937 XP_005246994 XM_005246938 XP_005246995 XM_011512100 XP_011510402 NM_001127183 NP_001120655 NM_001127184 NP_001120656 NM_001202515 NP_001189444 NM_001202516 NP_001189445 NM_001202517 NP_001189446 NM_001202518 NP_001189447 NM_001202519 NP_001189448 NM_001308042 NP_001294971 NM_001308043 NP_001294972 NM_003879 NP_003870
Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. {ECO:0000269|PubMed:9880531}.
Tissue Specificity Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
Top Pathways Apoptosis, Chagas disease (American trypanosomiasis), TNF signaling pathway

FLIP (D16A8) Rabbit mAb - 8510 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16A8
Western blot analysis of extracts from various cell lines using FLIP (D16A8) Rabbit mAb.

FLIP Antibody - 3210 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa (human), PC12 (rat) and NIH/3T3 (mouse) cell lines, using FLIP Antibody.

FLIP L (5D8) - sc-136160 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
FLIP<sub>L</sub> (5D8): sc-136160. Western blot analysis of FLIP<sub>L</sub> expression in mouse kidney tissue extract (A), MCF7 whole cell lysate (B), and rat spleen tissue extract (C).

FLIP L (H-150) - sc-8346 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FLIP<sub>L</sub> (H-150): sc-8346. Western blot analysis of FLIP<sub>L</sub> expression in Jurkat whole cell lysate.

FLIP L (F-20) - sc-7111 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FLIP<sub>L</sub> (F-20): sc-7111. Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.

FLIP L (C-19) - sc-7108 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FLIP<sub>L</sub> (C-19): sc-7108. Western blot analysis of FLIP<sub>L</sub> expression in MIA PaCa-2 (A), A431 (B), TF-1 (C) and JAR (D) whole cell lysates.

FLIP S/L (G-11) - sc-5276 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FLIP<sub>S/L</sub> (G-11): sc-5276. Western blot analysis of FLIP<sub>S/L</sub> expression in SW480 (A), Jurkat (B) and CTLL-2 (C) whole cell lysates.

FLIP S/L (H-202) - sc-8347 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FLIP<sub>S/L</sub> (H-202): sc-8347. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Anti-FLIP antibody - ab8421 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/mlLane 2 : Anti-FLIP antibody (ab8421) at 2 µg/mlLane 1 : K562 cell lysateLane 2 : K562 cell lysate

Anti-FLIP antibody - ab6144 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FLIP antibody (ab6144) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : Jurkat whole cell lysateLane 3 : K562 whole cell lysate

Anti-FLIP antibody - ab185822 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling FLIP with ab185822 at 1/200. Magnification: 200x.

Anti-FLIP antibody - ab56531 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-FLIP antibody - ab84283 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FLIP antibody (ab84283) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FLIP antibody - ab76870 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-FLIP antibody (ab76870) at 1/500 dilution + human placenta at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-FLIP antibody - ab93037 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Anti-FLIP antibody (ab93037) at 1/500 dilution + Human placenta tissue lysate at 50 µg

Anti-FLIP antibody - ab9945 from Abcam

Prices $359.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-FLIP antibody [2F12] - ab118643 from Abcam

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2F12
ab118643, at 10 µg/ml, staining FLIP in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry.

Anti-FLIP antibody [5D8] - ab81617 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5D8
All lanes : Anti-FLIP antibody [5D8] (ab81617) at 1/500 dilutionLane 1 : Cell lysates of mouse kidneyLane 2 : Cell lysates of MCF7Lane 3 : Cell lysates of rat spleenLysates/proteins at 40 µg per lane.SecondaryGoat anti-mouse secondary antibody conjugated to HRP developed using the ECL technique

Anti-FLIP antibody [7A3D12] - ab175423 from Abcam

Host Mouse
Clonality Monoclonal
Clone 7A3D12
Anti-FLIP antibody [7A3D12] (ab175423) at 1/500 dilution + FLIP recombinant protein

Anti-FLIP antibody [EPR8438(2)] - ab167409 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8438(2)
ab167409 showing +ve staining in Human ovarian carcinoma.

Anti-FLIP antibody [EPR8438(2)] (Alexa Fluor® 488) - ab197533 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8438(2)
ab197533 staining FLIP in A673 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197533 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FLIP antibody [EPR8438(2)] (Alexa Fluor® 647) - ab196849 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8438(2)
ab196849 staining FLIP in A673 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196849 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2 µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FLIP delta + gamma antibody - ab8423 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FLIP delta + gamma antibody (ab8423) at 1/1000 dilutionLane 1 : HeLa total cell lysateLane 2 : Jurkat total cell lysateLane 3 : THP1 total cell lysateLane 4 : A431 total cell lysateLane 5 : K562 total cell lysateLane 6 : NIH3T3 cellsObserved band size : 25,35 kDa (why is the actual band size different from the predicted?)

Anti-CFLAR / FLIP Antibody (aa210-460) - LS-C293194 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant CFLAR / FLIP.

Anti-CFLAR / FLIP Antibody (aa152-182) - LS-C168708 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CFLAR Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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