Anti-FLIP delta + gamma antibody

• Supplier: Abcam
• Catalog: ab8423
• Prices: $370.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide: QKSLKDPSNNFRMITPYAH , corresponding to C terminal amino acids 191/209 of Human FLIP delta + gamma
• Blocking Peptide: Human FLIP peptide
• Description: Rabbit Polyclonal
• Gene: CFLAR
• Conjugate: Unconjugated

Product images

All lanes : Anti-FLIP delta + gamma antibody (ab8423) at 1/1000 dilutionLane 1 : HeLa total cell lysateLane 2 : Jurkat total cell lysateLane 3 : THP1 total cell lysateLane 4 : A431 total cell lysateLane 5 : K562 total cell lysateLane 6 : NIH3T3 cellsObserved band size : 25,35 kDa (why is the actual band size different from the predicted?)

ab8423 at 2µg/ml staining FLIP delta + gamma in Hela cells by ICC/IF

All lanes : Anti-FLIP delta + gamma antibody (ab8423) at 1/5000 dilutionLane 1 : TF-1 whole cell lysateLane 2 : TF-1 whole cell lysateLane 3 : TF-1 whole cell lysateLane 4 : Jurkat whole cell lysateLysates/proteins at 30 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/4000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 30,44,98 kDa (why is the actual band size different from the predicted?)Exposure time : 2 minutesThis image is courtesy of an anonymous AbreviewSee Abreview

Product References

HO-1 underlies resistance of AML cells to TNF-induced apoptosis. - HO-1 underlies resistance of AML cells to TNF-induced apoptosis.

Rushworth SA, MacEwan DJ. Blood. 2008 Apr 1;111(7):3793-801.