Anti-FLNB antibody (ab97457) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Anti-FLNB antibody (ab97457) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
ab97547, at a 1/200 dilution, staining FLNB in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
Immunofluorescence analysis of Human trabecular meshwork cells, staining FLNB with ab97457. Left panel: FLNB staining alone. Right panel: FLNB staining (red) merged with F-actin staining (green). Cells were treated with dexamethasone, before fixing in paraformaldehyde and permeabilizing wih 0.2% Triton X-100. Cells were incubated with primary antibody (10 µg/ml) and AlexaFluor®488-conjugated phalloidin. FLNB staining was detected using an AlexaFluor®546-conjugated goat anti-rabbit IgG.