Anti-Flotillin 1 antibody

• Supplier: Abcam
• Catalog: ab41927
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC IHC-P ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Rat, Dog, Human, Broad species
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Flotillin 1
• Description: Rabbit Polyclonal
• Gene: FLOT1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Flotillin 1 antibody (ab41927) at 1 µg/mlLane 1 : Lung (Human) Tissue LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ab41927 (1/200) staining Flotillin 1 in HeLa cells (green). Cells were fixed with Methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview

ab41927 (1/100) staining Flotilin 1 in paraffin embedded - Human (anal cancer), Mouse (brain) and Rat (spleen) tissues. Tissue sections underwent paraformaldehyde fixation, heat mediated antigen retrieval using Citrate Buffer pH 6.0 and were blocked using sequential peroxidase and protein block (prediluted). Please refer to abreview for further experimental details.See Abreview

Flotillin 1 immunoprecipitation from Human HeLa whole cell lysate using ab41927. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.Lane 1 : anti-Flotillin 2 IP (using non-Abcam antibody)Lane 2 : anti-Flotillin 2 IP (using ab41927)Lane 3 : Input (HeLa cell lysate) Western blot detection : mouse anti-Flotillin 1 antibody + HRP donkey anti-mouse polyclonal secondary antibodySee Abreview

All lanes : Anti-Flotillin 1 antibody (ab41927) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab41927 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41927 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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