FLOT1

Gene Symbol FLOT1
Entrez Gene 10211
Alt Symbol -
Species Human
Gene Type protein-coding
Description flotillin 1
Other Description flotillin-1|integral membrane component of caveolae
Swissprots O75955 Q9UNV8 Q9UHW1 B4DVY7 Q969J8
Accessions BAB63320 BAC54934 BAE78620 BAF31269 EAX03325 EAX03326 EAX03327 O75955 AF085357 AAD40192 AF089750 AAC35387 AF117234 AAF17215 AK000816 AK222530 BAD96250 AK301291 BAG62849 BC001146 AAH01146 BC128154 AAI28155 BT007077 AAP35740 CR456946 CAG33227 DQ890639 ABM92256 DQ893815 ABM84741 XM_005248780 XP_005248837 XM_005248781 XP_005248838 XM_005272759 XP_005272816 XM_005272760 XP_005272817 XM_005274909 XP_005274966 XM_005274910 XP_005274967 XM_005275335 XP_005275392 XM_005275336 XP_005275393 XM_005275502 XP_005275559 XM_005275503 XP_005275560 XM_006714947 XP_006715010 XM_006725465 XP_006725528 XM_006725672 XP_006725735 XM_006725971 XP_006726034 XM_006726072 XP_006726135 NM_005803 NP_005794
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Subcellular Location Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome. Endosome. Note=Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Top Pathways Insulin signaling pathway

Flotillin-1 Antibody - 3253 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines and tissues using Flotillin-1 Antibody.

Flotillin-1 (C-20) - sc-16642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Flotillin-1 (C-7) - sc-133153 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Flotillin-1 (C-7): sc-133153. Western blot analysis of Flotillin-1 expression in K-562 (A), A-431 (B) and Jurkat (C) whole cell lysates.

Flotillin-1 (K-19) - sc-16640 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Flotillin-1 (K-19): sc-16640. Western blot analysis of Flotillin-1 expression in A-431 (A), Jurkat (B) and induced adipose differentiated 3T3-L1 (C) whole cell lysates.

Flotillin-1 (H-104) - sc-25506 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Flotillin-1 (H-104): sc-25506. Western blot analysis of Flotillin-1 expression in 3T3-L1 (A) and A-431 (B) whole cell lysates.

Anti-Flotillin 1 antibody - ab41927 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Flotillin 1 antibody (ab41927) at 1 µg/mlLane 1 : Lung (Human) Tissue LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Flotillin 1 antibody - ab50671 from Abcam

Prices $382.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Indirect Immunofluorescence. Human 293T cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 10 µg/mL Anti-Flotillin 1. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.

Anti-Flotillin 1 antibody - ab13493 from Abcam

Prices $390.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ICC/IF image of ab13493 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells. The cells were then incubated with the antibody ab13493 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Flotillin 1 antibody - ab123512 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of Flotillin 1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab123512 at 6&microg/mg lysate for IP (Lane 1). For WB detection an anti-Flotillin 1 antibody which recognizes an downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-Flotillin 1 antibody [EP446Y] - ab78178 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP446Y
Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/500 dilution + K562 cell lysate

Anti-Flotillin 1 antibody [EPR6041] - ab133497 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6041
All lanes : Anti-Flotillin 1 antibody [EPR6041] (ab133497) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : A431 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Flotillin 1 antibody - N-terminal - ab139100 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Flotillin 1 antibody - N-terminal (ab139100) at 1/100 dilution + HepG2 cell line lysate at 35 µg

Anti-FLOT1 / Flotillin 1 Antibody - LS-C193972 from LifeSpan Bioscience

Prices $905.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa189-238) - LS-C152067 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FLOT1 / Flotillin 1 antibody Western Blot of HeLa.

Anti-FLOT1 / Flotillin 1 Antibody (aa104-133, APC) - LS-C207036 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa104-133, Biotin) - LS-C207037 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa104-133, FITC) - LS-C207038 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa104-133, Azide-free, HRP) - LS-C207039 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa104-133, PE) - LS-C207040 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa104-133, AP) - LS-C208939 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (Internal, clone EP446Y) - LS-C49646 from LifeSpan Bioscience

Prices $470.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP446Y
Western blot analysis on K562 cell lysate using anti-Flotillin-1 antibody, dilution 1:500

Anti-FLOT1 / Flotillin 1 Antibody (aa104-133) - LS-C159664 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
FLOT1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The FLOT1 antibody detected the FLOT1 protein (arrow).

Anti-FLOT1 / Flotillin 1 Antibody (aa150-200) - LS-C286163 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa377-427) - LS-C286164 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FLOT1 / Flotillin 1 Antibody (aa126-225) - LS-C307575 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
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