Anti-Flotillin 2 antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Immunohistochemical analysis of paraffin-embedded Lung CA, using ab96507 at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
Flotillin 2 immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.Lane 1 : anti-Flotillin 2 IP (using non-Abcam antibody)Lane 2 : anti-Flotillin 2 IP (using ab96507)Lane 3 : Input (HeLa cell lysate)Lane 4 : Supernatant IP (using non-Abcam antibody)Lane 5 : Supernatant IP (using ab96507) Western blot detection : mouse anti-Flotillin 2 antibody + HRP donkey anti-mouse polyclonal secondary antibodySee Abreview