All lanes : Anti-Flotillin 2 antibody [EPR14128(B)] (ab181988) at 1/4000 dilutionLane 1 : 293 cell lysateLane 2 : Hela cell lysateLane 3 : A375 cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation of Hela cell lysate labeling Flotillin 2 using ab181988 at a 1/40 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.