All lanes : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/2000 dilution (purified)Lane 1 : lysateLane 2 : lysateLysates/proteins at 1/1000 dilution per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/2000 dilution (purified) + JAR cell lysate at 20 µgSecondaryHRP goat ant-rabbit (H+L) at 1/1000 dilution
Lanes 1 - 2 : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/10000 dilution (purified)Lanes 3 - 4 : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 10000 (purified)Lane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLane 3 : HEK293 cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryLanes 1 - 2 : HRP goat anti-rabbit (H+L) at 1/1000 dilutionLanes 3 - 4 : HRP goat anti-rabbit at 1/1000 dilution
All lanes : Anti-Folate Binding Protein antibody [EPR4708(2)] (ab125030) at 1/1000 dilution (Unpurified)Lane 1 : JAR cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLane 4 : 293T cell lysateLane 5 : A549 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit at 1/2000 dilution