All lanes : Anti-Folylpolyglutamate synthase antibody (ab175849) at 1/1000 dilutionLane 1 : K562 cell line lysateLane 2 : A2058 cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling Folylpolyglutamate synthase with ab175849 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells labeling Folylpolyglutamate synthase with ab175849 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.