Anti-Folylpolyglutamate synthase antibody [EPR11064] (ab184564) at 1/50000 dilution + K562 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Folylpolyglutamate synthase antibody [EPR11064] (ab184564) at 1/10000 dilutionLane 1 : JAR cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometry analysis of Folylpolyglutamate synthase expression in paraformaldehyde-fixed HeLa cells using ab184564 at 1/60 dilution (red) and a rabbit IgG as negative control (green). Secondary antibody: Goat anti-rabbit IgG (FITC).