Anti-FOXA1 antibody
| Name | Anti-FOXA1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55178 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 367-473 of Human FOXA1 |
| Description | Mouse Monoclonal |
| Gene | FOXA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
FOXA1 antibody (ab55178) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.
ab55178 at 10 ug/ml staining FOXA1 in human HepG2 cells by Immunocytochemistry/ Immunofluorescence.
![Overlay histogram showing HepG2 cells stained with ab55178 (red line). The cells were fixed with 4 % paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55178, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/20217_FOXA1-Primary-antibodies-ab55178-2.jpg)
Overlay histogram showing HepG2 cells stained with ab55178 (red line). The cells were fixed with 4 % paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55178, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Forkhead box A1 (FOXA1) is a key mediator of insulin-like growth factor I (IGF-I) - Forkhead box A1 (FOXA1) is a key mediator of insulin-like growth factor I (IGF-I)
Potter AS, Casa AJ, Lee AV. J Cell Biochem. 2012 Jan;113(1):110-21.
Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated - Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated
Oloumi A, Maidan M, Lock FE, Tearle H, McKinney S, Muller WJ, Aparicio SA, Dedhar S. Breast Cancer Res. 2010;12(3):R38.