FOXA1

Gene Symbol FOXA1
Entrez Gene 3169
Alt Symbol HNF3A, TCF3A
Species Human
Gene Type protein-coding
Description forkhead box A1
Other Description HNF-3-alpha|HNF-3A|TCF-3A|forkhead box protein A1|hepatocyte nuclear factor 3-alpha|transcription factor 3A
Swissprots B2R9H6 P55317 B7ZAP5 Q9H2A0
Accessions AAD51979 AAG40847 EAW65844 P55317 AK304119 BAG65019 AK313785 BAG36523 AK316360 BAH14731 AM392863 CAL37741 AM393239 CAL38117 AM393310 CAL38188 AM393369 CAL38247 BC033890 AAH33890 BM970983 BU860564 DA705947 U39840 AAB06493 NM_004496 NP_004487
Function Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activit
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00089, ECO:0000269|PubMed:15987773, ECO:0000269|PubMed:16331276}.
Tissue Specificity Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. {ECO:0000269|PubMed:12234996, ECO:0000269|PubMed:15987773, ECO:0000269|PubMed:16331276}.

HNF-3α (H-120) - sc-22841 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HNF-3α (H-120): sc-22841. Western blot analysis of HNF-3α expression in Hep G2 (A) and DU 145 (B) nuclear extracts.

HNF-3α (T-20) - sc-9186 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HNF-3α (T-20): sc-9186. Western blot analysis of HNF-3α expression in Hep G2 whole cell lysate (A) and Hep G2 nuclear extract (B).

HNF-3α (Q-6) - sc-101058 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HNF-3α (Q-6): sc-101058. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate tissue showing nuclear localization.

HNF-3α/β (E-4) - sc-377033 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HNF-3α/β (E-4): sc-377033. Western blot analysis of HNF-3β expression in non-transfected: sc-117752 (A) and human HNF-3β transfected: sc-176240 (B) 293T whole cell lysates.

HNF-3α/β (C-20) - sc-6553 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HNF-3α/β (C-20): sc-6553. Western blot analysis of HNF-3β expression in non-transfected: sc-117752 (A) and human HNF-3β transfected: sc-176240 (B) 293T whole cell lysates.

Anti-FOXA1 antibody - ab55178 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
FOXA1 antibody (ab55178) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.

Anti-FOXA1 antibody - ab151522 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FOXA1 with ab151522 at 1/1000 dilution. Lower image shows cells co-stained with Hoechst 33342.

Anti-FOXA1 antibody - ab115279 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Human Breast formalin-fixed, paraffin-embedded tissue stained with ab115279 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-FOXA1 antibody - ab123780 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-FOXA1 antibody (ab123780) at 1/100 dilution + 293 cell line lysates at 35 µg

Anti-FOXA1 antibody [2F83] - ab40868 from Abcam

Prices $390.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F83
Ab40868 staining Mouse fetal lung tissue at 1:200 dilution by Immunohistochemistry paraffin embedded sections. FOXA1 was visualised by DAB (dark red colour) and counterstained with Nuclear Fast Red (pink colour). Magnification 20x.

Anti-FOXA1 antibody [3A8] - ab117857 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A8
All lanes : Anti-FOXA1 antibody [3A8] (ab117857) at 1/1000 dilutionLane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY FOXA1 cDNA for 48 hrs Lysates/proteins at 5 µg per lane.

Anti-FOXA1 antibody [3C1] - ab125091 from Abcam

Prices $385.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3C1
Anti-FOXA1 antibody [3C1] (ab125091) at 1/500 dilution + HepG2 cell lysate

Anti-FOXA1 antibody [EP4131] - ab109760 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP4131
All lanes : Anti-FOXA1 antibody [EP4131] (ab109760) at 1/1000 dilutionLane 1 : HepG2 lysateLane 2 : PC-3 lysateLane 3 : SW480 lysateLane 4 : Human colon cancer lysateLysates/proteins at 10 µg per lane.

Anti-FOXA1 antibody [EPR10881] - ab170933 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10881
All lanes : Anti-FOXA1 antibody [EPR10881] (ab170933) at 1/1000 dilutionLane 1 : HepG2 lysatesLane 2 : SW480 lysatesLane 3 : Mouse lung lysatesLane 4 : Rat lung lysatesLysates/proteins at 10 µg per lane.

Anti-FOXA1 antibody [EPR10881] (Alexa Fluor® 488) - ab197235 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10881
Overlay histogram showing HepG2 cells stained with ab197235 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197235, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HepG2 fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-FOXA1 antibody [EPR10881] (Alexa Fluor® 647) - ab196908 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10881
ab196908 staining FOXA1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196908 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FOXA1 antibody [EPR10881-14] - ab173287 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10881-14
ab173287 showing -ve staining in Human brain tissue. 1:1000 dilution of ab. Paraffin-embedded sections.

Anti-FOXA1 antibody [SP88] - ab99892 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP88
Flow cytometric analysis of rabbit anti-FOXA1 (SP88) antibody ab99892 in HepG2 cells (green) compared to negative control of rabbit IgG (blue).

Anti-FOXA1 antibody [SP88], prediluted - ab99893 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Monoclonal
Clone SP88
Staining of Human FOXA1 in a Formalin/PFA-fixed paraffin-embedded section of Human Prostate using prediluted ab99893.

Anti-FOXA1 antibody - ChIP Grade - ab23738 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-FOXA1 antibody - ChIP Grade (ab23738) at 1 µg/ml + Human heart tissue lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Anti-FOXA1 antibody - ChIP Grade - ab5089 from Abcam

Prices $400.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Goat polyclonal to FOXA1 antibody (ab5089) was used in a ChIP assay to detect in vivo binding of FOXA1/HNF3a to the promoter of the TFF1 gene in MCF-7 cells. The cells were fixed in 1% formaldehyde for 15 minutes at room temperature and ChIP was performed using 6µg/ml ab5089. Real-time PCR was used to quantify the enrichment of the TFF1 promoter in the FOXA1 ChIP'd DNA, using the input DNA as the reference. The results indicate that there is an 18-fold enrichment of the TFF1 promoter in the DNA ChIp'd with ab5089.  This image was submitted as part of a review.See Abreview

Anti-FOXA1 Antibody (clone 4F6) IHC-plus™ - LS-B4356 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4F6
Anti-FOXA1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4356 concentration 5 ug/ml.

Anti-FOXA1 Antibody (clone 3C1) IHC-plus™ - LS-B6101 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3C1
Anti-FOXA1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6101 concentration 5 ug/ml.

Anti-FOXA1 Antibody (clone 2D7) IHC-plus™ - LS-B6099 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2D7
Anti-FOXA1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6099 concentration 5 ug/ml.

Anti-FOXA1 Antibody (clone 1B1) IHC-plus™ - LS-B6100 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1B1
Anti-FOXA1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6100 concentration 10 ug/ml.
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